用于生物素酶放射分析的生物素酰胺类似物的高效液相色谱分离。

High-performance liquid chromatographic separation of biotinylamide analogues used as substrates in biotinidase radioassays.

作者信息

Livaniou E, Roboti A, Kakabakos S E, Nyalala J, Evangelatos G P, Ithakissios D S

机构信息

Radioimmunochemistry Laboratory, Institute of Radioisotopes/Radiodiagnostic Products, NCSR Demokritos, Athens, Greece.

出版信息

J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):215-8. doi: 10.1016/0378-4347(94)00073-5.

DOI:10.1016/0378-4347(94)00073-5

PMID:7952032

Abstract

A simple, one-step protocol for synthesizing biotinylmono[125I]iodotyramine and biotinyldi[125I]iodotyramine, which are used as tracer substrates in biotinidase radioassays, is presented. This synthetic protocol uses reversed-phase HPLC for the isolation of the biotinylamide analogues from the reaction mixture. The HPLC method developed can potentially be applied to a scaled-up synthetic protocol for non-radioactive biotinylmono- and diiodotyramine. In addition, it can be used for the identification of the above-mentioned compounds.

摘要

本文介绍了一种简单的一步法合成生物素化单[¹²⁵I]碘酪胺和生物素化双[¹²⁵I]碘酪胺的方法，这两种化合物用作生物素酶放射测定中的示踪底物。该合成方法采用反相高效液相色谱从反应混合物中分离生物素酰胺类似物。所开发的高效液相色谱方法有可能应用于非放射性生物素化单碘和双碘酪胺的放大合成方案。此外，它可用于上述化合物的鉴定。