La Croix R, Pianezzola E, Strolin Benedetti M
Pharmacia-Farmitalia Carlo Erba, R&D, Pharmacokinetics and Metabolism Department, Milan, Italy.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):251-8. doi: 10.1016/0378-4347(94)00039-5.
A high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed for the determination in human plasma of the three main metabolites of selegiline (L-deprenyl): amphetamine, methamphetamine and norselegiline. The HPLC separation of the analytes was performed under isocratic conditions, after extraction from plasma and precolumn derivatization with 9-fluorenylmethyl chloroformate. The linearity, precision and accuracy of the method were evaluated; the limit of quantification for all three metabolites in plasma was 0.5 ng/ml.
