Balíková M, Vecerková J
Institute of Forensic Toxicology, 1st Medical Faculty, Charles University, Prague, Czech Republic.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):267-73. doi: 10.1016/0378-4347(94)00043-3.
In the later stages after intake, the important markers of cocaine abuse are its main metabolites in urine, benzoylecgonine and ecgonine methyl ester. The efficiency of the extraction of amphoteric benzoylecgonine together with cocaine from aqueous media by means of various solvents at various pH values and by means of a mixed solid phase was tested. The extraction of benzoylecgonine with diethyl ether is not efficient, whereas chloroform, dichloromethane or mixed solid-phase extraction give satisfactory results. The analytical strategy for the general chromatographic screening and identification of unknown drugs in biological samples based on diethyl ether extraction was modified to permit the sensitive detection of cocaine abuse also on the basis of benzoylecgonine. A complementary high-performance liquid chromatographic method with photodiode-array detection after solid-phase extraction was introduced for specific confirmation and determination of cocaine and benzoylecgonine.
在摄入后的后期阶段，可卡因滥用的重要标志物是其在尿液中的主要代谢产物，即苯甲酰爱康宁和芽子碱甲酯。测试了在不同pH值下使用各种溶剂以及通过混合固相从水介质中同时提取两性的苯甲酰爱康宁和可卡因的效率。用乙醚提取苯甲酰爱康宁效率不高，而氯仿、二氯甲烷或混合固相萃取则能给出令人满意的结果。基于乙醚萃取的生物样品中未知药物的一般色谱筛选和鉴定分析策略进行了修改，以便也能基于苯甲酰爱康宁灵敏地检测可卡因滥用情况。引入了一种在固相萃取后采用光电二极管阵列检测的互补高效液相色谱法，用于可卡因和苯甲酰爱康宁的特异性确证和测定。
