Polanuer B, Ivanov S, Sholin A
Institute for Genetics of Industrial Microorganisms, Moscow, Russian Federation.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):81-5. doi: 10.1016/0378-4347(94)00081-6.
A rapid and simple method for the determination of taurine (2-aminoethanesulphonic acid) in complex samples is described. It is based on the HPLC separation of the dinitrophenyl (DNP) derivative of taurine. The reaction conditions are selected to allow complete derivatization of taurine within 15 min. DNP-taurine samples are stable for at least 3 days. DNP-taurine was separated by reversed-phase liquid chromatography within 12 s. The recovery of taurine was 102 +/- 3% (S.D. = 2.5%, n = 6) and the detection limit was 10 pmol for taurine (signal-to-noise ratio of 10). The method was applied to the determination of taurine levels in different samples including marine products, infant formulas and fermentation media of different bacterial species.
本文描述了一种用于测定复杂样品中牛磺酸（2-氨基乙磺酸）的快速简便方法。该方法基于牛磺酸二硝基苯基（DNP）衍生物的高效液相色谱分离。选择反应条件以使牛磺酸在15分钟内完全衍生化。DNP-牛磺酸样品至少3天稳定。DNP-牛磺酸通过反相液相色谱在12秒内分离。牛磺酸的回收率为102±3%（标准差=2.5%，n=6），牛磺酸的检测限为10皮摩尔（信噪比为10）。该方法应用于测定不同样品中的牛磺酸水平，包括海产品、婴儿配方奶粉和不同细菌种类发酵培养基。
