通过手性相高效液相色谱法直接拆分环氧二十碳三烯酸对映体。

Direct resolution of epoxyeicosatrienoic acid enantiomers by chiral-phase high-performance liquid chromatography.

作者信息

Zhang J Y, Blair I A

机构信息

Department of Pharmacology, Vanderbilt University, Nashville, TN 37232-6602.

出版信息

J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):23-9. doi: 10.1016/0378-4347(94)80065-0.

DOI:10.1016/0378-4347(94)80065-0

PMID:7952074

Abstract

A chromatographic method was developed to separate enantiomers of four regioisomeric epoxyeicosatrienoic acids (EETs), which are cytochrome P-450 monooxygenase products of arachidonic acid. Enantiomers of individual epoxyeicosatrienoic acids were separated by chiral-phase HPLC using Chiralcel OD columns. In contrast to the pre-derivatization of EETs, the method is simple and convenient. It can be readily used in analytical studies and in preparative applications. The method can also be applied to the separation of methyl ester derivatives of epoxyeicosatrienoic acid enantiomers.

摘要

开发了一种色谱方法来分离四种区域异构环氧二十碳三烯酸（EETs）的对映体，这些EETs是花生四烯酸的细胞色素P-450单加氧酶产物。使用Chiralcel OD柱通过手性相高效液相色谱法分离各个环氧二十碳三烯酸的对映体。与EETs的预衍生化方法相比，该方法简单方便。它可容易地用于分析研究和制备应用中。该方法也可应用于环氧二十碳三烯酸对映体的甲酯衍生物的分离。