通过柱前非对映体衍生化对左旋和右旋肉碱进行高效液相色谱和毛细管电泳分析。
High-performance liquid chromatography and capillary electrophoresis of L- and D-carnitine by precolumn diastereomeric derivatization.
作者信息
De Witt P, Deias R, Muck S, Galletti B, Meloni D, Celletti P, Marzo A
机构信息
Department of Chemical Research, Sigma-Tau S.p.A., Pomezia, Rome, Italy.
出版信息
J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):67-73. doi: 10.1016/0378-4347(94)80071-5.
A new method for the simultaneous assay of D- and L-enantiomers of carnitine is described. The method is based on precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate [(+)FLEC] producing a diastereomeric derivative which can be detected both by UV absorbance and fluorescence detection. Also acyl esters of carnitine can be processed with this method, after alkaline hydrolysis. The D-enantiomer of carnitine and acylcarnitine can be detected at a concentration as low as 0.2% in the raw material and in pharmaceuticals. Assays can be carried out using an autoinjector either by HPLC or capillary electrophoresis (CE) because the derivative proved to be very stable. Its application is proposed for the routine assay of the enantiomeric excess of L-carnitine and their acyl esters in pharmaceutical products.
本文描述了一种同时测定肉碱D-和L-对映体的新方法。该方法基于用(+)-1-(9-芴基)乙基氯甲酸酯[(+)FLEC]进行柱前衍生化，生成一种非对映体衍生物，可通过紫外吸收和荧光检测进行检测。肉碱的酰基酯在碱性水解后也可用此方法处理。肉碱和酰基肉碱的D-对映体在原料和药物中的检测浓度低至0.2%。由于该衍生物非常稳定，因此可以使用自动进样器通过高效液相色谱(HPLC)或毛细管电泳(CE)进行测定。建议将其应用于药物产品中L-肉碱及其酰基酯对映体过量的常规测定。
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