采用芘-1-羰基氰化物柱前衍生化法通过液相色谱法测定肉碱。

Liquid chromatographic determination of carnitine by precolumn derivatization with pyrene-1-carbonyl cyanide.

作者信息

Kamata K, Takahashi M, Terasima K, Nishijima M

机构信息

Tokyo Metropolitan Research Laboratory of Public Health, Japan.

出版信息

J Chromatogr A. 1994 Apr 29;667(1-2):113-8. doi: 10.1016/0021-9673(94)89058-7.

DOI:10.1016/0021-9673(94)89058-7

PMID:8025624

Abstract

A method is described for the determination of carnitine chloride in pharmaceutical preparations by precolumn derivatization and high-performance liquid chromatography (HPLC). Carnitine chloride is derivatized by reaction with pyrene-1-carbonyl cyanide (PCC) in dimethyl sulphoxide. The PCC derivative of carnitine is then used for quantitative HPLC analysis on a cation-exchange column with fluorescence detection. The calibration graph was linear over a sample concentration range from 2.0 to 20 micrograms/ml; the limit of detection was 0.5 microgram/ml under the conditions studied. The proposed method was applied satisfactorily to the determination of carnitine chloride in pharmaceutical preparations.

摘要

本文描述了一种通过柱前衍生化和高效液相色谱法（HPLC）测定药物制剂中氯化肉碱的方法。氯化肉碱在二甲基亚砜中与芘-1-羰基氰化物（PCC）反应进行衍生化。然后，肉碱的PCC衍生物用于在阳离子交换柱上进行荧光检测的定量HPLC分析。在所研究的条件下，校准曲线在2.0至20微克/毫升的样品浓度范围内呈线性；检测限为0.5微克/毫升。所提出的方法令人满意地应用于药物制剂中氯化肉碱的测定。