Mizuguchi M, Ikeda K, Asada M, Mizutani S, Kamoshita S
Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Kodaira, Japan.
Brain Res. 1994 Jun 27;649(1-2):197-202. doi: 10.1016/0006-8993(94)91064-2.
To explore the role of the protooncogene bcl-2 in the prevention of programmed cell death in the nervous system, we investigated its expression in mouse neural cells in primary culture. The 26 kDa protein product, Bcl-2, was detected by immunocytochemistry and immunoblotting in cultured neurons, astrocytes and oligodendrocytes, but the immunoreactivity of microglial cells was not detectable by immunoblotting. The subcellular distribution of Bcl-2 was similar between in vivo (brain) and in vitro (culture) and between cultured neurons and astrocytes, while the content was higher in astrocytes than in neurons. The substantial expression of bcl-2 in primary cultured brain cells suggests that it has some physiological control in the brain over programmed cell death, which may be exerted not only in neurons but also in some glial cells such as astrocytes.
为了探究原癌基因bcl-2在预防神经系统程序性细胞死亡中的作用，我们研究了其在原代培养的小鼠神经细胞中的表达。通过免疫细胞化学和免疫印迹法在培养的神经元、星形胶质细胞和少突胶质细胞中检测到了26 kDa的蛋白产物Bcl-2，但通过免疫印迹法未检测到小胶质细胞的免疫反应性。Bcl-2的亚细胞分布在体内（脑）和体外（培养物）之间以及培养的神经元和星形胶质细胞之间相似，而星形胶质细胞中的含量高于神经元。bcl-2在原代培养的脑细胞中的大量表达表明，它在脑中对程序性细胞死亡具有某种生理控制作用，这种作用可能不仅作用于神经元，还作用于一些神经胶质细胞，如星形胶质细胞。
