在裸鼠体内表达表皮生长因子（EGF）受体的人卵巢癌异种移植物的植入和生长依赖于表皮生长因子（EGF）。
Implantation and growth of epidermal growth factor (EGF) receptor expressing human ovarian cancer xenografts in nude mice is dependent on EGF.
作者信息
Kurachi H, Morishige K, Adachi H, Adachi K, Tasaka K, Sawada M, Miyake A
机构信息
Department of Obstetrics and Gynecology, Osaka University, Medical School, Japan.
出版信息
Cancer. 1994 Dec 1;74(11):2984-90. doi: 10.1002/1097-0142(19941201)74:11<2984::aid-cncr2820741115>3.0.co;2-m.
BACKGROUND
The importance of epidermal growth factor (EGF) receptor-dependent growth has not been clarified for in vivo growth of primary human ovarian cancers.
METHODS
Seventeen primary human ovarian cancer tissue samples were examined for the presence of EGF receptors by a 125I-EGF-binding study. Three groups of mice were inoculated with EGF receptor expressing and not-expressing cancer tissues. The groups were as follows: control group, Sx group (mice that underwent sialoadenectomy; EGF depleted mice), and Sx+EGF (EGF-replaced) group. The ability of the inoculated tissues to implant and grow then was studied.
RESULTS
Of the 17 primary ovarian cancers, 12 expressed EGF receptors and 5 did not. Eight of 12 EGF-receptor expressing cancer tissues implanted and formed growing tumors in control animals. None implanted in the Sx animals. Epidermal growth factor receptor-expressing cancers implanted in Sx animals that received EGF administration. Two of five EGF receptor-negative ovarian cancers implanted and grew in both control and Sx animals.
CONCLUSION
Growth of EGF receptor-expressing primary human ovarian cancers may be dependent on EGF in vivo.
背景
表皮生长因子（EGF）受体依赖性生长对于原发性人类卵巢癌的体内生长的重要性尚未阐明。
方法
通过¹²⁵I-EGF结合研究检测了17份原发性人类卵巢癌组织样本中EGF受体的存在情况。将三组小鼠接种表达和不表达EGF受体的癌组织。分组如下：对照组、Sx组（接受涎腺切除术的小鼠；EGF耗竭小鼠）和Sx + EGF（EGF替代）组。然后研究接种组织的植入和生长能力。
结果
17例原发性卵巢癌中，12例表达EGF受体，5例不表达。12例表达EGF受体的癌组织中有8例在对照动物中植入并形成生长的肿瘤。在Sx动物中无一例植入。表达表皮生长因子受体的癌组织植入接受EGF给药的Sx动物体内。5例EGF受体阴性的卵巢癌中有2例在对照和Sx动物中均植入并生长。
结论
表达EGF受体的原发性人类卵巢癌的生长在体内可能依赖于EGF。
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