大肠杆菌O83:K24:H31的O83特异性多糖的结构

Structure of the O83-specific polysaccharide of Escherichia coli O83:K24:H31.

作者信息

Jann B, Shashkov A S, Hahne M, Kochanowski H, Jann K

机构信息

Max-Planck-Institut für Immunbiologie, Freiburg, Germany.

出版信息

Carbohydr Res. 1994 Aug 17;261(2):215-22. doi: 10.1016/0008-6215(94)84018-0.

DOI:10.1016/0008-6215(94)84018-0

PMID:7954513

Abstract

The polysaccharide moiety of the O83 antigen (lipopolysaccharide, LPS) consists of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-glucuronic acid in the molar ratios 1:2:1:1. Methylation analysis of the polysaccharide and derived oligosaccharides as well as one- and two-dimensional 1H and 13C NMR spectroscopy of the polysaccharide at pD 1 and 6 showed that the O83 polysaccharide has the primary structure-->6)-alpha-D-Glc p-(1-->4)-beta-D-Glc pA-(1-->6)-beta-D-Gal p-(1-->4)-beta-D- Gal p-(1-->4)-beta-D-Glc pNAc-(1-->.

摘要

O83抗原（脂多糖，LPS）的多糖部分由D-葡萄糖、D-半乳糖、2-乙酰氨基-2-脱氧-D-葡萄糖和D-葡萄糖醛酸组成，摩尔比为1:2:1:1。对该多糖及其衍生的寡糖进行甲基化分析，以及在pD 1和6条件下对该多糖进行一维和二维1H和13C核磁共振光谱分析，结果表明O83多糖具有以下一级结构：→6)-α-D-Glc p-(1→4)-β-D-Glc pA-(1→6)-β-D-Gal p-(1→4)-β-D-Gal p-(1→4)-β-D-Glc pNAc-(1→ 。