Boyd J M, Malstrom S, Subramanian T, Venkatesh L K, Schaeper U, Elangovan B, D'Sa-Eipper C, Chinnadurai G
Institute for Molecular Virology, St. Louis University Health Sciences Center, Missouri 63110.
Cell. 1994 Oct 21;79(2):341-51. doi: 10.1016/0092-8674(94)90202-x.
Adenovirus E1B 19 kDa protein protects against cell death induced by viral infection and certain external stimuli. The Bcl-2 protein can functionally substitute for the E1B 19 kDa protein. To identify cellular targets for the 19 kDa protein, we used the two-hybrid screen in yeast. We have isolated cDNAs for three different proteins, designated Nip1, Nip2, and Nip3, that interact with the 19 kDa protein. Mutational analysis indicates that these proteins do not associate with 19 kDa mutants defective in suppression of cell death, suggesting a correlation between interaction of these proteins and suppression of cell death. These proteins also associate with discrete sequence motifs in the Bcl-2 protein that are homologous to motifs of the 19 kDa protein. Our results suggest that two diverse proteins, the E1B 19 kDa and the Bcl-2 proteins, promote cell survival through interaction with a common set of cellular proteins.
腺病毒E1B 19千道尔顿蛋白可保护细胞免受病毒感染及某些外部刺激诱导的细胞死亡。Bcl-2蛋白在功能上可替代E1B 19千道尔顿蛋白。为了鉴定19千道尔顿蛋白的细胞靶点，我们在酵母中进行了双杂交筛选。我们分离出了三种不同蛋白质的cDNA，分别命名为Nip1、Nip2和Nip3，它们可与19千道尔顿蛋白相互作用。突变分析表明，这些蛋白质不与在抑制细胞死亡方面存在缺陷的19千道尔顿突变体相关联，这表明这些蛋白质的相互作用与细胞死亡抑制之间存在关联。这些蛋白质还与Bcl-2蛋白中与19千道尔顿蛋白基序同源的离散序列基序相关联。我们的结果表明，两种不同的蛋白质，即E1B 19千道尔顿蛋白和Bcl-2蛋白，通过与一组共同的细胞蛋白相互作用来促进细胞存活。
