Taira Z, Matsumoto M, Ishida S, Ichikawa T, Sakiya Y
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Chem Pharm Bull (Tokyo). 1994 Aug;42(8):1556-61. doi: 10.1248/cpb.42.1556.
The aggregation of DNA caused by coralyne was studied by spectroscopic, viscosity and electric birefringence measurements. Aggregation was markedly enhanced over a narrow range of coralyne to DNA phosphate ratio and then followed by precipitation. The electric birefringence measurements indicated that the ratio at the maximal aggregation varied depending on the concentration of coralyne, finally reaching 1:1 at higher concentrations. The particles (type I) for such enhanced aggregation were estimated to be prolate ellipsoids 1700-4000 A in length with a diameter of 1400-4000 A. At higher coralyne concentrations, another particle (type II) was formed which was a thick rod-like particle 1700-4000 A in length with a diameter of 120-210 A. These dimensions indicate that type I and II particles consist respectively of several tens of thousands and some hundreds of molecules of DNA. On the other hand, berberine did not produce such a marked aggregation of DNA, and the result was a thick rod-like particle 1700-4000 A in length with a diameter of 300-1000 A. The enhancement by coralyne and berberine is discussed in terms of intermolecular interactions.
通过光谱、粘度和电双折射测量研究了珊瑚碱引起的DNA聚集。在珊瑚碱与DNA磷酸根比例的狭窄范围内，聚集显著增强，随后发生沉淀。电双折射测量表明，最大聚集时的比例随珊瑚碱浓度而变化，在较高浓度下最终达到1:1。这种增强聚集的颗粒（I型）估计是长度为1700 - 4000 Å、直径为1400 - 4000 Å的长椭球体。在较高的珊瑚碱浓度下，形成了另一种颗粒（II型），它是长度为1700 - 4000 Å、直径为120 - 210 Å的粗棒状颗粒。这些尺寸表明I型和II型颗粒分别由数万个和数百个DNA分子组成。另一方面，黄连素不会使DNA产生如此明显的聚集，结果是形成了长度为1700 - 4000 Å、直径为300 - 1000 Å的粗棒状颗粒。从分子间相互作用的角度讨论了珊瑚碱和黄连素的增强作用。
