Wang X, Qiu L, He Y, Mao S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(2):100-3.
Hemocytes from Oncomelania hupensis hemolymph were studied in vitro. Spreading amoebocytes were the most common cell type. The average sizes of amoebocytes from Anhui and Yunnan were 8.42 +/- 0.73 x 7.46 +/- 0.52 microns and 7.63 +/- 0.65 x 6.85 +/- 0.54 microns, respectively. The nucleus was round in shape, and eosinophile granular inclusions were found in the endocytoplasm. Contacting with slide, the cells became flattened with extending pseudopodia. The ability of amoebocytes from snails of both localities to phagocytose sheep red blood cells was confirmed in vitro. The method to collect hemolymph from Oncomelania hupensis was developed, and the promise of this method in studying host-parasite relationship as well as epidemiological investigation of schistosomiasis japonica in the mainland of China was discussed.
对湖北钉螺血淋巴中的血细胞进行了体外研究。伸展性变形细胞是最常见的细胞类型。来自安徽和云南的变形细胞平均大小分别为8.42±0.73×7.46±0.52微米和7.63±0.65×6.85±0.54微米。细胞核呈圆形,胞质内可见嗜酸性颗粒包涵体。细胞与载玻片接触后会变扁平并伸出伪足。体外证实了两个地区钉螺的变形细胞吞噬绵羊红细胞的能力。开发了从湖北钉螺采集血淋巴的方法,并讨论了该方法在研究宿主-寄生虫关系以及中国大陆日本血吸虫病流行病学调查中的前景。