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在β-2-噻吩丙氨酸存在的情况下对致病性革兰氏阴性细菌属的鉴别。

Differentiation of pathogenic, gram-negative, bacterial genera in the presence of beta-2-thienylalanine.

作者信息

Brown K J, Lines D R

出版信息

Can J Microbiol. 1976 Dec;22(12):1673-9. doi: 10.1139/m76-248.

DOI:10.1139/m76-248
PMID:795531
Abstract

Described in a fast screening assay for aid in the differentiation of bacterial genera. Salmonella is separated from Escherichia and Shigella, as a generic group on the basis of an inhibition assay incorporating beta-2-thienylalanine, and using a defined medium. The assay is also usable for confirmation of taxonomic differences between genera. The inhibition reaction, readable as an 'all-or-none' phenomenon, follows a dose-response relationship for cultures affected by beta-2-thienylalanine, and is unaffected by the growth phase of the culture.

摘要

在一种用于辅助细菌属鉴别分型的快速筛选测定法中有所描述。基于包含β-2-噻吩丙氨酸的抑制测定法并使用特定培养基,沙门氏菌作为一个属类与大肠杆菌和志贺氏菌区分开来。该测定法也可用于确认不同属之间的分类差异。这种抑制反应呈现为“全或无”现象,对于受β-2-噻吩丙氨酸影响的培养物遵循剂量反应关系,且不受培养物生长阶段的影响。

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Differentiation of pathogenic, gram-negative, bacterial genera in the presence of beta-2-thienylalanine.在β-2-噻吩丙氨酸存在的情况下对致病性革兰氏阴性细菌属的鉴别。
Can J Microbiol. 1976 Dec;22(12):1673-9. doi: 10.1139/m76-248.
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引用本文的文献

1
The assay on a defined medium of the effects of beta-2-thienylalanine on the growth of anaerobic bacterial isolates from phenylketonuric patients.在特定培养基上检测β-2-噻吩丙氨酸对苯丙酮尿症患者厌氧细菌分离株生长的影响。
Med Microbiol Immunol. 1980 Feb;168(1):11-24. doi: 10.1007/BF02121648.
2
Colonization by Salmonella typhimurium and Shigella flexneri III of the gastrointestinal tract of mice treated with beta-2-thienylalanine and streptomycin.用β-2-噻吩丙氨酸和链霉素处理的小鼠胃肠道被鼠伤寒沙门氏菌和弗氏志贺氏菌III型定植。
Antonie Van Leeuwenhoek. 1979;45(4):531-46. doi: 10.1007/BF00403653.
3
Effects of increasing Mg++ ion concentration on the PKU monitoring assay.
Experientia. 1979 Apr 15;35(4):462-3. doi: 10.1007/BF01922707.