Venkataraman V, O'Mahony P J, Manzcak M, Jones G
Molecular and Cellular Section, School of Biological Sciences, University of Kentucky, Lexington 40506.
Dev Genet. 1994;15(5):391-400. doi: 10.1002/dvg.1020150502.
Juvenile hormone (JH) is a major hormone regulating insect development. We have obtained a cDNA and a genomic clone for juvenile hormone esterase (JHE), the enzyme that is involved in the degradation of juvenile hormone and which is critical for insect development. Analysis of the regulation of JHE during the final larval stadium in the cabbage looper, Trichoplusia ni, showed that the JHE mRNA levels are maximal on days 2 and 4 of the final stadium. Nuclear run-on analyses demonstrated that changes in JHE mRNA levels are primarily due to changes in the transcription rate of the gene, which may be a single copy in the genome. Treatment with a JH analog resulted in induction of JHE gene transcription, which could be detected within three hours after treatment. Salient features present in the 5' flanking region of this JH-sensitive gene are presented, including the presence of sequences closely resembling binding sites for members of the family of nuclear receptors. This report is the first direct demonstration, by nuclear run-on analysis, of JH induction of gene transcription.
保幼激素（JH）是调节昆虫发育的主要激素。我们已获得保幼激素酯酶（JHE）的cDNA和基因组克隆，该酶参与保幼激素的降解，对昆虫发育至关重要。对甘蓝夜蛾（Trichoplusia ni）末龄幼虫期JHE的调控分析表明，末龄幼虫期第2天和第4天JHE mRNA水平最高。核转录分析表明，JHE mRNA水平的变化主要是由于该基因转录速率的变化，该基因在基因组中可能是单拷贝的。用JH类似物处理导致JHE基因转录的诱导，在处理后三小时内即可检测到。本文介绍了该JH敏感基因5'侧翼区域的显著特征，包括存在与核受体家族成员结合位点非常相似的序列。本报告是通过核转录分析首次直接证明JH诱导基因转录。
