Eisenhauer K M, McCue P M, Nayden D K, Osawa Y, Roser J F
Department of Animal Science, University of California, Davis 95616.
Domest Anim Endocrinol. 1994 Jul;11(3):291-8. doi: 10.1016/0739-7240(94)90020-5.
Stallion testes secrete large amounts of estrogens, but the cellular location of the enzyme that converts androgens to estrogens, cytochrome P450 aromatase, has not been determined. The goal of the present study was to immunocytochemically localize stallion testicular aromatase using a polyclonal antibody generated against human placental cytochrome P450 aromatase. Testes were obtained from 12 stallions from 2 to 23 years of age, during both the breeding and non-breeding seasons. Immunoreactivity was confined to the Leydig cells in all testes examined. No immunostaining was observed in the Sertoli or germ cells. Heterogeneity in the level of immunostaining among individual Leydig cells was observed. The results of this study indicate that in postpubertal, adult, and aged stallions, testicular aromatase is located in Leydig cells.
种马的睾丸会分泌大量雌激素，但将雄激素转化为雌激素的酶——细胞色素P450芳香化酶的细胞定位尚未确定。本研究的目的是使用针对人胎盘细胞色素P450芳香化酶产生的多克隆抗体，通过免疫细胞化学方法定位种马睾丸中的芳香化酶。在繁殖季节和非繁殖季节，从12匹年龄在2至23岁的种马获取睾丸。在所检查的所有睾丸中，免疫反应性局限于睾丸间质细胞。在支持细胞或生殖细胞中未观察到免疫染色。观察到单个睾丸间质细胞之间免疫染色水平存在异质性。本研究结果表明，在青春期后、成年和老龄种马中，睾丸芳香化酶位于睾丸间质细胞中。
