Wang D, Yang J Y, Wang B L
Pediatric Department, First Teaching Hospital, Beijing Medical University.
Chin Med J (Engl). 1994 Jun;107(6):437-9.
To identify the role of circulating immune factor(s) in the development of proteinuria, the supernatant of peripheral blood mononuclear cells (PBMC) were obtained from 15 patients with steroid-responsive nephrotic syndrome, and its effect on the synthesis of sulfated compound by cultured rat glomerular epithelial cell (GEC) were studied by 35S, 3H-leucine dual-isotope labelling technique. The result showed that the supernatant of PBMC from 9 patients without steroid treatment increased 35S uptake in GEC (P < 0.01), while there was no significant difference in 35S uptake between the 6 patients treated with prednisone (1.5-2.0 mg/kg.d-1) and 6 normal controls.
为了确定循环免疫因子在蛋白尿发生中的作用，从15例激素反应性肾病综合征患者中获取外周血单个核细胞（PBMC）的上清液，并用35S、3H-亮氨酸双同位素标记技术研究其对培养的大鼠肾小球上皮细胞（GEC）硫酸化化合物合成的影响。结果显示，9例未经激素治疗患者的PBMC上清液使GEC对35S的摄取增加（P<0.01），而6例接受泼尼松（1.5 - 2.0mg/kg·d-1）治疗的患者与6例正常对照者的GEC对35S的摄取无显著差异。
