Li X, Wang H, Jiang Y
Cancer Research Institute, China Medical University, Shenyang.
Zhonghua Zhong Liu Za Zhi. 1994 May;16(3):181-3.
A human pancreatic cancer cell line(JF305) was established from a pancreatic adenocarcinoma xenograft in nude mice. These cells have passed 103 passages in more than 1 year. The plating efficiency was 30.6%, and doubling time was 49.5 hours. Chromosomal analysis showed a human karyotype with a chromosome number between 66-130. JF305 cells exhibited epithelial morphologic features resembling the structure of the original tumor, and they grew to born tumor when inoculated again into nude mice. With immunohistochemical staining, the nuclei of JF305 cells were positive for p53. Therefore, JF305 offer a good model for the study of human pancreatic adenocarcinoma and P53 gene mutation.
一种人胰腺癌细胞系（JF305）是从裸鼠体内的胰腺腺癌异种移植瘤中建立的。这些细胞在1年多的时间里传代了103次。平板接种效率为30.6%，倍增时间为49.5小时。染色体分析显示为人类核型，染色体数目在66 - 130之间。JF305细胞表现出类似于原发肿瘤结构的上皮形态特征，再次接种到裸鼠体内时会生长成肿瘤。通过免疫组织化学染色，JF305细胞的细胞核p53呈阳性。因此，JF305为研究人类胰腺腺癌和P53基因突变提供了一个良好的模型。
