睾丸生殖细胞中特异性表达的cDNA克隆的分离与鉴定
Isolation and characterization of cDNA clones specifically expressed in testicular germ cells.
作者信息
Tanaka H, Yoshimura Y, Nishina Y, Nozaki M, Nojima H, Nishimune Y
机构信息
Department of Science for Laboratory Animal Experimentation, Osaka University, Japan.
出版信息
FEBS Lett. 1994 Nov 21;355(1):4-10. doi: 10.1016/0014-5793(94)01155-9.
PMID:7957958
Abstract
We have cloned cDNAs involved in germ cell-specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild-type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled.
摘要
我们已经克隆了参与生殖细胞特异性表达的cDNA。为此，通过从野生型睾丸cDNA中减去来自突变型睾丸支持细胞的cDNA，构建了一个消减cDNA文库。对mRNA表达的详细分析表明，与克隆的cDNA相对应的基因仅在睾丸中表达，并受发育调控。
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