Iwata Y, Hanada H, Takahashi M, Shigekawa M
Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan.
FEBS Lett. 1994 Nov 21;355(1):65-8. doi: 10.1016/0014-5793(94)01174-5.
We have investigated the distribution of the sarcolemmal Ca2+ transporters in hamster and dog ventricular myocytes by immunocytochemical and membrane fractionation techniques. The data suggest that the DHP receptor alpha 1 subunit and the Na+/Ca2+ exchanger are present in surface sarcolemma as well as T-tubule membranes located at the cardiac dyads. Compared with these Ca2+ transporters, the sarcolemmal Ca(2+)-ATPase is much less abundant in the latter fraction. Thus the sarcolemmal Ca(2+)-ATPase seems to be located predominantly in surface sarcolemma.
我们通过免疫细胞化学和膜分离技术研究了仓鼠和犬心室肌细胞中肌膜钙转运体的分布。数据表明，二氢吡啶受体α1亚基和钠钙交换体存在于表面肌膜以及位于心脏二联体处的横管膜中。与这些钙转运体相比，肌膜钙ATP酶在后一部分中的含量要少得多。因此，肌膜钙ATP酶似乎主要位于表面肌膜。
