Ricarová B, Bilińska B, Viklický V, Stokłosowa S
Institute of Molecular Genetics, Czech Academy of Sciences, Prague.
Folia Histochem Cytobiol. 1994;32(2):107-12.
Formation of lymphocyte rosettes around unstimulated and/or LH/hCG-treated Leydig cells in culture as well as in suspension was investigated. The ability of Leydig cells to interact with lymphocytes was positively correlated with their steroidogenic activity, as measured by means of a histochemical test for delta (5)3 beta-HSD activity and by radioimmunoassay for androgen level in culture media. In order to rule out nonspecific binding of lymphocytes, rosette specificity tests were performed. Time-dependent dynamics of cell-cell interaction was also followed. A phenomenon resembling phagocytosis of the adhered lymphocytes was observed after 4 h of co-culture. Morphology of this interaction was examined under light microscope and transmission electron microscope.
研究了在培养物中以及悬浮状态下，未受刺激的和/或经促黄体生成素/人绒毛膜促性腺激素处理的睾丸间质细胞周围淋巴细胞花环的形成情况。睾丸间质细胞与淋巴细胞相互作用的能力与其类固醇生成活性呈正相关，这通过对δ(5)3β - 羟基类固醇脱氢酶活性的组织化学检测以及对培养基中雄激素水平的放射免疫测定来衡量。为了排除淋巴细胞的非特异性结合，进行了花环特异性测试。还跟踪了细胞间相互作用的时间依赖性动态变化。共培养4小时后，观察到一种类似于吞噬黏附淋巴细胞的现象。在光学显微镜和透射电子显微镜下检查了这种相互作用的形态。
