Murase T
Department of Obstetrics and Gynecology, Teikyo University, School of Medicine, Tokyo.
Nihon Naibunpi Gakkai Zasshi. 1994 Mar 20;70(2):95-104. doi: 10.1507/endocrine1927.70.2_95.
To investigate the influences of intrauterine stress on the aromatase activity (AA) in the perinatal rat's brain, mothers underwent 3 grades of stress: saline injection stress (S), light-heat-mild restraint stress (M), and forced immobilization stress (F). The aromatase activities in the offsprings' hypothalamus and amygdala were determined on day 19 of gestation and on the 1st day after birth. In addition, serum levels of testosterone (T) and androstenedione (A) were measured. In males, perinatal levels of T and A decreased with every grade of prenatal stress. In females, T levels were not affected by prenatal stress. Fetal hypothalamic AA on the 19th day of gestation decreased significantly only in male fetuses of group M and F. Neonatal hypothalamic AA on day 1 after birth decreased only in males of all stress groups. Meanwhile, fetal and neonatal AA in the amygdala did not show any changes in either sex. These results indicate that intrauterine stress depletes both serum androgens and hypothalamic AA in the critical period for sexual differentiation of the brain, which mainly regulates the hypothalamic sex differentiation.
为研究宫内应激对围产期大鼠大脑芳香化酶活性（AA）的影响，将孕鼠分为3种应激等级：生理盐水注射应激（S）、光-热-轻度束缚应激（M）和强迫固定应激（F）。在妊娠第19天和出生后第1天测定仔鼠下丘脑和杏仁核中的芳香化酶活性。此外，还测定了血清睾酮（T）和雄烯二酮（A）水平。在雄性大鼠中，围产期T和A水平随产前应激等级的升高而降低。在雌性大鼠中，T水平不受产前应激的影响。仅在M组和F组的雄性胎儿中，妊娠第19天的胎儿下丘脑AA显著降低。出生后第1天，仅在所有应激组的雄性仔鼠中，新生儿下丘脑AA降低。同时，杏仁核中的胎儿和新生儿AA在两性中均未显示任何变化。这些结果表明，宫内应激在大脑性别分化的关键时期消耗了血清雄激素和下丘脑AA，这主要调节下丘脑的性别分化。
