Fan N C, Jeung E B, Peng C, Olofsson J I, Krisinger J, Leung P C
Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, Canada.
Mol Cell Endocrinol. 1994 Jul;103(1-2):R1-6. doi: 10.1016/0303-7207(94)90087-6.
The cDNA encoding the gonadotropin-releasing hormone (GnRH) receptor has recently been cloned and characterized in several species, including human. To determine the structure of the gene encoding the human GnRH receptor, we have screened a human genomic library and isolated seven positive clones, using cDNA probes derived from a human pituitary cDNA library. The isolated genomic clone contains the entire protein coding region of the GnRH receptor which is distributed between three exons and spans over 18.9 kb. Sequence analysis and restriction endonuclease mapping revealed the presence of two introns of 4.2 and 5.0 kb, respectively, both located within the open reading frame, designating the human GnRH receptor gene to the intron-containing class of the G-protein coupled receptor superfamily. Genomic Southern blot analysis indicated the presence of a single copy of the gene encoding for the GnRH receptor within the human genome. Using DNA from human-hamster somatic hybrid cell lines, the GnRH receptor gene was assigned to human chromosome 4, by means of PCR. The present study represents the first report on the GnRH receptor gene and its partial characterization should facilitate further investigation of the mechanisms by which expression of this gene is regulated.
编码促性腺激素释放激素（GnRH）受体的cDNA最近已在包括人类在内的多个物种中被克隆和鉴定。为了确定编码人类GnRH受体的基因结构，我们使用源自人垂体cDNA文库的cDNA探针筛选了一个人类基因组文库，并分离出7个阳性克隆。分离出的基因组克隆包含GnRH受体的整个蛋白质编码区，该编码区分布在三个外显子之间，跨度超过18.9 kb。序列分析和限制性内切酶图谱显示分别存在4.2 kb和5.0 kb的两个内含子，两者均位于开放阅读框内，将人类GnRH受体基因归入含内含子的G蛋白偶联受体超家族类别。基因组Southern印迹分析表明人类基因组中存在编码GnRH受体的基因的单拷贝。通过PCR，利用人-仓鼠体细胞杂种细胞系的DNA，将GnRH受体基因定位于人类4号染色体。本研究是关于GnRH受体基因的首次报道，其部分特征鉴定应有助于进一步研究该基因表达调控的机制。
