c-myc的过表达先于编码二氢叶酸还原酶的基因扩增。
Overexpression of c-myc precedes amplification of the gene encoding dihydrofolate reductase.
作者信息
Mai S
机构信息
Basel Institute for Immunology, Switzerland.
出版信息
Gene. 1994 Oct 21;148(2):253-60. doi: 10.1016/0378-1119(94)90696-3.
PMID:7958952
Abstract
An experimentally inducible model system was generated in which Chinese hamster ovary cells (CHO-9) were stably transfected with an inducible c-myc cDNA. The induction of c-myc in these transfectants is followed by the enhanced binding of c-Myc/Max-containing protein complexes to 5'flanking E-box sequences of the gene encoding dihydrofolate reductase (DHFR). Moreover, DHFR is transiently amplified. The inappropriate overproduction of the oncoprotein, therefore, seems to plays a role in induced DHFR amplification.
摘要
构建了一个实验性诱导模型系统，其中中国仓鼠卵巢细胞（CHO-9）被稳定转染了可诱导的c-myc cDNA。这些转染细胞中c-myc的诱导伴随着含c-Myc/Max的蛋白复合物与编码二氢叶酸还原酶（DHFR）基因的5'侧翼E-box序列的结合增强。此外，DHFR会短暂扩增。因此，癌蛋白的不适当过量产生似乎在诱导的DHFR扩增中起作用。
相似文献
1
Overexpression of c-myc precedes amplification of the gene encoding dihydrofolate reductase.c-myc的过表达先于编码二氢叶酸还原酶的基因扩增。
Gene. 1994 Oct 21;148(2):253-60. doi: 10.1016/0378-1119(94)90696-3.
2
C-myc overexpression facilitates radiation-induced DHFR gene amplification.C-myc过表达促进辐射诱导的二氢叶酸还原酶（DHFR）基因扩增。
Int J Radiat Biol. 1997 Feb;71(2):167-75. doi: 10.1080/095530097144283.
3
4
Expression of amplified cDNA and genomic sequences encoding human interleukin 2 in Chinese hamster ovary cells.在中国仓鼠卵巢细胞中编码人白细胞介素2的扩增cDNA和基因组序列的表达。
J Biochem. 1987 Jul;102(1):123-31. doi: 10.1093/oxfordjournals.jbchem.a122023.
5
Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome.鉴定和分析中国仓鼠卵巢细胞基因组中外源 Dhfr 扩增区域附近的特定染色体区域。
J Biosci Bioeng. 2010 May;109(5):504-11. doi: 10.1016/j.jbiosc.2009.10.019. Epub 2009 Nov 18.
6
c-Myc overexpression associated DHFR gene amplification in hamster, rat, mouse and human cell lines.
Oncogene. 1996 Jan 18;12(2):277-88.
7
Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.人组织型纤溶酶原激活剂与小鼠二氢叶酸还原酶序列在中国仓鼠卵巢细胞中的共扩增与共表达。
Mol Cell Biol. 1985 Jul;5(7):1750-9. doi: 10.1128/mcb.5.7.1750-1759.1985.
8
Transfection with a cDNA encoding a Ser31 or Ser34 mutant human dihydrofolate reductase into Chinese hamster ovary and mouse marrow progenitor cells confers methotrexate resistance.将编码Ser31或Ser34突变型人二氢叶酸还原酶的cDNA转染到中国仓鼠卵巢细胞和小鼠骨髓祖细胞中可赋予甲氨蝶呤抗性。
Gene. 1994 Feb 25;139(2):269-74. doi: 10.1016/0378-1119(94)90768-4.
9
Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.缩写的小鼠二氢叶酸还原酶基因在培养的仓鼠细胞中的表达。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6522-6. doi: 10.1073/pnas.79.21.6522.
10
Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells.姐妹染色单体融合引发中国仓鼠细胞中二氢叶酸还原酶基因的扩增。
Genes Dev. 1993 Apr;7(4):605-20. doi: 10.1101/gad.7.4.605.
引用本文的文献
1
Low expression of miR-let-7a promotes cell growth and invasion through the regulation of c-Myc in oral squamous cell carcinoma.miR-let-7a 低表达通过调节 c-Myc 促进口腔鳞状细胞癌的细胞生长和侵袭。
Cell Cycle. 2020 Aug;19(15):1983-1993. doi: 10.1080/15384101.2020.1786633. Epub 2020 Jun 28.
2
The Dual Roles of MYC in Genomic Instability and Cancer Chemoresistance.MYC在基因组不稳定和癌症化疗耐药性中的双重作用
Genes (Basel). 2017 Jun 7;8(6):158. doi: 10.3390/genes8060158.
3
Modeling and Targeting MYC Genes in Childhood Brain Tumors.儿童脑肿瘤中MYC基因的建模与靶向治疗
Genes (Basel). 2017 Mar 23;8(4):107. doi: 10.3390/genes8040107.
4
Functional interactions among members of the MAX and MLX transcriptional network during oncogenesis.肿瘤发生过程中MAX和MLX转录网络成员之间的功能相互作用。
Biochim Biophys Acta. 2015 May;1849(5):484-500. doi: 10.1016/j.bbagrm.2014.05.016. Epub 2014 May 22.
5
c-MYC-induced genomic instability.c-MYC诱导的基因组不稳定。
Cold Spring Harb Perspect Med. 2014 Apr 1;4(4):a014373. doi: 10.1101/cshperspect.a014373.
6
Unbalanced replication as a major source of genetic instability in cancer cells.不平衡复制作为癌细胞遗传不稳定的主要来源。
Am J Blood Res. 2012;2(3):160-9. Epub 2012 Oct 20.
7
Prevalence of c-myc expression in breast lesions associated with microcalcifications detected by routine mammography.通过常规乳腺X线摄影检测到的与微钙化相关的乳腺病变中c-myc表达的患病率。
Sao Paulo Med J. 2009 May;127(2):66-70. doi: 10.1590/s1516-31802009000200003.
8
c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleus.c-Myc通过端粒和间期细胞核中的染色体重塑诱导染色体重排。
Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9613-8. doi: 10.1073/pnas.0407512102. Epub 2005 Jun 27.
9
c-Myc-induced extrachromosomal elements carry active chromatin.c-Myc诱导的染色体外元件携带活性染色质。
Neoplasia. 2003 Mar-Apr;5(2):110-20. doi: 10.1016/s1476-5586(03)80002-7.
10
The impact of p53 loss on murine plasmacytoma development.p53缺失对小鼠浆细胞瘤发生发展的影响。
Chromosome Res. 2002;10(3):239-51. doi: 10.1023/a:1015200307448.
Zotero 插件