Woo H H, Brigham L A, Hawes M C
Department of Plant Pathology, University of Arizona, Tucson 85721.
Gene. 1994 Oct 21;148(2):369-70. doi: 10.1016/0378-1119(94)90715-3.
The complementary DNA (PsU BC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.
已克隆出代表豌豆泛素结合酶（UBC）编码mRNA的互补DNA（PsU BC4）。编码区长度为444个核苷酸（nt），能够指定一个由148个氨基酸（aa）组成、等电点为7.95的16.5 kDa蛋白质。推导的氨基酸序列与拟南芥AtUBC8 - 12家族和番茄ERT17显示出97%的同一性，与酵母ScUBC4和ScUBC5以及黑腹果蝇DmUBC4显示出80%的同一性。迄今为止在UBC中发现的活性位点半胱氨酸（Cys85）在豌豆序列中也保守。
