Miloux B, Lupker J H
Sanofi Recherche, Centre de Labège, France.
Gene. 1994 Nov 18;149(2):341-4. doi: 10.1016/0378-1119(94)90173-2.
An expression system combining a unit for the expression of the gene of interest reinforced by the hepatitis B virus X transactivator and a selectable gene weakened by the insertion of an A+T-rich sequence derived from the 3'-untranslated region of the granulocyte-macrophage colony-stimulating factor mRNA is described. This vector allows rapid one-step isolation of highly productive Chinese hamster ovary clones.
描述了一种表达系统，其结合了由乙型肝炎病毒X反式激活因子增强的目的基因表达单元和通过插入源自粒细胞-巨噬细胞集落刺激因子mRNA 3'-非翻译区的富含A+T序列而弱化的选择基因。该载体允许快速一步分离高产的中国仓鼠卵巢克隆。
