Qin K N, Khanna M, Cheng K C
Department of Pediatrics, Cornell University Medical College, New York, NY 10021.
Gene. 1994 Nov 18;149(2):357-61. doi: 10.1016/0378-1119(94)90176-7.
The structure and sequence of the gene (DD/BABP) encoding a human dihydrodiol dehydrogenase/bile acid-binding protein (DD/BABP) were determined by analysis of genomic clones. Several overlapping clones containing parts of the gene were isolated from a lambda EMBL3 SP6/T7 library and characterized by restriction mapping and DNA sequencing. The gene spans approx. 16kb and consists of nine exons. The sizes of the exons range from 77 to 167 bp, whereas the intron sizes range from 375 to 3430 bp. The transcription start point (tsp) is located at -56 bp of the first ATG codon, as determined by primer extension. Neither a TATA box nor a CAT box was found upstream from the tsp. Southern blot analysis of the human genomic DNA, using the cDNA as the probe, revealed several additional hybridizing DNA bands, suggesting the existence of multiple related genes.
通过对基因组克隆的分析，确定了编码人二氢二醇脱氢酶/胆汁酸结合蛋白（DD/BABP）的基因（DD/BABP）的结构和序列。从λEMBL3 SP6/T7文库中分离出几个包含该基因部分片段的重叠克隆，并通过限制性图谱分析和DNA测序进行了表征。该基因跨度约为16kb，由九个外显子组成。外显子大小范围为77至167bp，而内含子大小范围为375至3430bp。通过引物延伸确定转录起始点（tsp）位于第一个ATG密码子的-56bp处。在tsp上游未发现TATA盒或CAT盒。用人基因组DNA进行Southern印迹分析，以cDNA为探针，发现了几条额外的杂交DNA条带，表明存在多个相关基因。
