Dirks W, Schaper F, Hauser H
Department of Human and Animal Cell Culture, DSM-German Collection of Microorganisms and Cell Cultures, Braunschweig.
Gene. 1994 Nov 18;149(2):389-90. doi: 10.1016/0378-1119(94)90187-2.
A hybrid promoter which generates large amounts of mRNAs with transcription start points (tsp) differing in one nt, both in mammalian cells and in vitro, was constructed by integrating the promoter of bacteriophage T7 in between the TATA box and the tsp of the retroviral myeloproliferative sarcoma virus (MPSV) long terminal repeat (LTR). This promoter was designed for sequence and secondary structure studies of 5' untranslated regions (UTR) with respect to mRNA stability and translatability.
通过将噬菌体T7启动子整合到逆转录病毒骨髓增殖性肉瘤病毒（MPSV）长末端重复序列（LTR）的TATA框和转录起始点（tsp）之间，构建了一种杂合启动子，该启动子在哺乳动物细胞和体外均可产生大量转录起始点相差一个核苷酸的mRNA。该启动子专为研究5'非翻译区（UTR）的序列和二级结构对mRNA稳定性和可翻译性的影响而设计。
