Sgherzi M R, Fabbri G, Bonati M, Maietta Latessa A, Segre A, De Vita D, De Leo V, Genazzani A D, Petraglia F, Genazzani A R
Department of Obstetrics and Gynecology, University of Modena, Italy.
Gynecol Obstet Invest. 1994;38(1):60-4. doi: 10.1159/000292448.
The aim of the present study was to optimize the use of serum procollagen type I carboxy-terminal propeptide (PICP) as a possible marker of postmenopausal-376lted changes of bone metabolism. Postmenopausal (n = 20) and healthy fertile young (n = 4) women were studied after informed consent. The postmenopausal women were subdivided in 4 groups: (1) nontreated; (2) treated with estrogen-progestogen replacement therapy; (3) treated with calcitonin, or (4) with kidney or liver diseases. Blood samples were collected at 15-min time intervals for 4, 6 or 8 h. Serum concentration of PICP was measured by radioimmunoassay, in duplicate at two different dilutions. In postmenopausal women mean +/- SEM serum PICP levels were slightly but nonsignificantly higher than in fertile women. Serum PICP levels in estrogen-progestogen or calcitonin-treated women were significantly lower than in non-treated postmenopausal women. Episodic changes of circulating PICP level were observed in fertile and postmenopausal women. The pulses of serum PICP levels did not show significant differences among the groups of women studied. The present study showed that the measurement of serum PICP levels is a useful marker for investigating the changes of bone metabolism. In particular, low PICP levels in postmenopausal women under steroid hormone or calcitonin treatment in part reflect the changes of bone turnover. The pulses of serum PICP levels during a time interval suggest that collagen metabolism in women undergoes a rapid turnover.
本研究的目的是优化血清I型前胶原羧基末端前肽（PICP）作为绝经后相关骨代谢变化潜在标志物的应用。在获得知情同意后，对绝经后女性（n = 20）和健康育龄年轻女性（n = 4）进行了研究。绝经后女性被分为4组：（1）未治疗组；（2）接受雌激素 - 孕激素替代治疗组；（3）接受降钙素治疗组；或（4）患有肾脏或肝脏疾病组。每隔15分钟采集一次血样，共采集4、6或8小时。通过放射免疫分析法在两种不同稀释度下对PICP的血清浓度进行双份测定。绝经后女性的平均±标准误血清PICP水平略高于育龄女性，但差异无统计学意义。接受雌激素 - 孕激素或降钙素治疗的女性血清PICP水平显著低于未治疗的绝经后女性。在育龄和绝经后女性中均观察到循环PICP水平的间歇性变化。所研究的女性组之间血清PICP水平的波动无显著差异。本研究表明，测定血清PICP水平是研究骨代谢变化的有用标志物。特别是，绝经后女性在接受类固醇激素或降钙素治疗时PICP水平较低，部分反映了骨转换的变化。在一个时间段内血清PICP水平的波动表明女性的胶原蛋白代谢周转迅速。
