Tashiro T
Department of Neurosurgery, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1994 Jul;69(4):742-9.
Tumor necrosis factor (TNF)-alpha gene were transfected into lymphokine-activated killer (LAK) cells generated from peripheral blood lymphocytes incubated with recombinant interleukin-2 by means of novel liposomes with a positive charge on their surface. The cells secreted significant amounts of TNF-alpha into the culture medium and exhibited reinforcement of cytotoxicity toward a human glioma cell line (U251-SP), being three times more cytotoxic than nontransfected LAK cells. The mechanism for the reinforcement of cytotoxicity is considered to involve not only an increase in TNF-alpha secretion from LAK cells but also its expression on their surface. Intratumoral or intrathecal injection of LAK cells transfected with the TNF-alpha gene may be useful for the treatment of patients with malignant glioma.
通过表面带正电荷的新型脂质体，将肿瘤坏死因子（TNF）-α基因转染到由外周血淋巴细胞与重组白细胞介素-2孵育产生的淋巴因子激活的杀伤细胞（LAK细胞）中。这些细胞向培养基中分泌大量的TNF-α，并对人胶质瘤细胞系（U251-SP）表现出细胞毒性增强，其细胞毒性是非转染LAK细胞的三倍。细胞毒性增强的机制被认为不仅涉及LAK细胞TNF-α分泌的增加，还涉及其在细胞表面的表达。瘤内或鞘内注射转染了TNF-α基因的LAK细胞可能对恶性胶质瘤患者的治疗有用。
