Bastmeyer M, Jeserich G, Stuermer C A
Faculty of Biology, University of Konstanz, Germany.
Glia. 1994 Aug;11(4):300-14. doi: 10.1002/glia.440110403.
In light of the striking differences between oligodendrocytes of the optic nerve/tract of adult goldfish and their mammalian counterparts, a further characterization of goldfish oligodendrocytes was performed. A comparison with Schwann cells was included because fish optic nerve/tract-derived oligodendrocytes bear remarkable similarities to this type of glial cell. Fish optic nerve/tract-derived oligodendrocytes that had differentiated into 04 and 6D2-positive cells and thus expressed early myelin marker molecules were found to incorporate BrdU and to divide in vitro over a period of weeks. For the induction of more advanced markers of myelinogenesis such as the CNS-specific myelin protein 36K, oligodendrocytes required extensive contact with axons. Other agents, such as fetal calf or carp serum, substrate components, or forscolin failed, however, to induce 36K expression. 04/6D2-positive oligodendrocytes could be distinguished from fish 6D2-positive Schwann cells derived from cranial nerves by their antigenic phenotype: Schwann cells but not oligodendrocytes exhibited the low affinity NGF receptor. While both cell types carry the cell adhesion molecules NCAM, E 587 antigen, and the L2/HNK-1 epitope, only Schwann cells possess a further adhesion molecule, Neurolin.
鉴于成年金鱼视神经/视束中的少突胶质细胞与其哺乳动物对应细胞之间存在显著差异，对金鱼少突胶质细胞进行了进一步的特性分析。研究中还将其与施万细胞进行了比较，因为源自金鱼视神经/视束的少突胶质细胞与这种神经胶质细胞类型有显著相似之处。已分化为04和6D2阳性细胞从而表达早期髓鞘标记分子的源自金鱼视神经/视束的少突胶质细胞，在体外数周内被发现能掺入溴脱氧尿苷并进行分裂。为了诱导更高级的髓鞘形成标记物，如中枢神经系统特异性髓鞘蛋白36K，少突胶质细胞需要与轴突广泛接触。然而，其他物质，如胎牛血清或鲤鱼血清、底物成分或福斯高林，均未能诱导36K的表达。04/6D2阳性少突胶质细胞可通过其抗原表型与源自颅神经的金鱼6D2阳性施万细胞区分开来：施万细胞而非少突胶质细胞表现出低亲和力神经生长因子受体。虽然这两种细胞类型都携带细胞粘附分子NCAM、E 587抗原和L2/HNK-1表位，但只有施万细胞拥有另一种粘附分子，即神经粘附素。
