Ben Ali H, Guerin J F, Pinatel M C, Mathieu C, Boulieu D, Tritar B
Laboratoire de Cytogénétique et Biologie de la Reproduction, Faculté de Médecine de Sousse, Tunisia.
Int J Androl. 1994 Jun;17(3):121-6. doi: 10.1111/j.1365-2605.1994.tb01230.x.
Unfertilized oocytes from an in-vitro fertilization programme were stored in different saline solutions and then utilized in a zona binding assay (ZBA). The four saline solutions tested were identical with regard to the capacity of the zona to bind spermatozoa provided by healthy donors. Spermatozoa from 150 infertile patients were tested in the ZBA. The number of spermatozoa bound to the zona correlated positively with sperm concentration, the percentage sperm motility and the percentage of morphologically normal spermatozoa. The population was then divided into two groups according to the level of alpha-glucosidase activity, an epididymal marker. The average number of spermatozoa bound to the zona was diminished in the group with low alpha-glucosidase activity, even when considering strictly equivalent classes of sperm concentration, motility and morphology, respectively.
来自体外受精项目的未受精卵母细胞被保存在不同的盐溶液中，然后用于透明带结合试验（ZBA）。所测试的四种盐溶液在由健康供体提供的透明带结合精子的能力方面是相同的。在ZBA中对150名不育患者的精子进行了测试。与透明带结合的精子数量与精子浓度、精子活力百分比以及形态正常的精子百分比呈正相关。然后根据附睾标志物α-葡萄糖苷酶活性水平将人群分为两组。即使分别考虑严格等效的精子浓度、活力和形态类别，α-葡萄糖苷酶活性低的组中与透明带结合的精子平均数量也会减少。
