Yoon S C, Park J M, Jang H S, Shinn K S, Bahk Y W
Department of Radiology, Catholic University Medical College, Seoul, Korea.
Int J Radiat Oncol Biol Phys. 1994 Nov 15;30(4):873-8. doi: 10.1016/0360-3016(94)90363-8.
Captopril, an inhibitor of angiotensin I converting enzyme, has been shown to modify radiation damage and prevent radiation injury of normal tissue in rats and pigs. The present study was carried out to determine whether captopril would reduce radiation changes in the proximal small bowel in mice.
Mice were subjected to whole body irradiation with 9 Gy or 15 Gy. Captopril was administered in drinking water at a regimen of 62.5 mg/kg/day (captopril group I) and 125 mg/kg/day (captopril group II), continuously from 7 days before irradiation to the end of each designed experiment. The jejunal damage was evaluated microscopically by crypt count per circumference and by histologic damage grading.
Crypt number in the sham-irradiated control was 133 +/- 6.8/circumference. In both captopril group I and II, crypt numbers and histologic scores were not significantly different from those in the normal group. The 9 Gy and 15 Gy radiation alone groups showed significantly lower crypt counts and histologic scores compared with the sham-irradiated control group (p < 0.05). The groups exposed to 9 Gy radiation plus captopril I and II showed significantly higher crypt counts and lower histologic damage scores on the third day, and lower histologic damage scores on the fifth day compared with the 9 Gy radiation alone group (p < 0.05). The 15 Gy radiation plus captopril I and II groups had significantly higher crypt counts and lower histologic damage scores on the third day than those of the 15 Gy radiation alone group (p < 0.05). All mice of the 15 Gy radiation group succumbed to intestinal radiation death.
Our results suggest that captopril provides protection from acute radiation damage to the jejunal mucosa in mice.
卡托普利是一种血管紧张素I转换酶抑制剂，已被证明可改变辐射损伤并预防大鼠和猪正常组织的辐射损伤。本研究旨在确定卡托普利是否能减轻小鼠近端小肠的辐射变化。
小鼠接受9 Gy或15 Gy的全身照射。从照射前7天至每个设计实验结束，以62.5 mg/kg/天（卡托普利I组）和125 mg/kg/天（卡托普利II组）的方案通过饮用水给予卡托普利。通过每周长的隐窝计数和组织学损伤分级对空肠损伤进行显微镜评估。
假照射对照组的隐窝数为133±6.8/周长。在卡托普利I组和II组中，隐窝数和组织学评分与正常组相比均无显著差异。与假照射对照组相比，单纯9 Gy和15 Gy辐射组的隐窝计数和组织学评分显著降低（p<0.05）。与单纯9 Gy辐射组相比，接受9 Gy辐射加卡托普利I和II组在第三天的隐窝计数显著更高，组织学损伤评分更低，在第五天的组织学损伤评分也更低（p<0.05）。15 Gy辐射加卡托普利I和II组在第三天的隐窝计数显著高于单纯15 Gy辐射组，组织学损伤评分更低（p<0.05）。15 Gy辐射组的所有小鼠均死于肠道辐射死亡。
我们的结果表明，卡托普利可保护小鼠空肠黏膜免受急性辐射损伤。
