Mackey B M, Cross D, Park S F
Institute of Food Research, Reading Laboratory, UK.
J Appl Bacteriol. 1994 Aug;77(2):149-54. doi: 10.1111/j.1365-2672.1994.tb03058.x.
Bacterial luciferase was used to investigate the relationship between the thermostability of a cytoplasmic reporter molecule and cellular heat resistance. The luciferase activity of Vibrio fischeri was expressed in strains of Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Brochothrix thermosphacta following transformation with plasmid pSP13 carrying the luxAB genes. The thermostability of intracellular luciferase varied depending on the organism in which it was expressed, but was not related to the cellular heat resistance of the different organisms. Addition of xylitol to the heating medium protected against loss of viability and inactivation of intracellular luciferase. Glycerol also protected against loss of viability but was less effective at preventing thermal denaturation of luciferase.
利用细菌荧光素酶研究细胞质报告分子的热稳定性与细胞耐热性之间的关系。在用携带luxAB基因的质粒pSP13转化后，费氏弧菌的荧光素酶活性在大肠杆菌、鼠伤寒沙门氏菌、单核细胞增生李斯特菌和嗜热栖热放线菌菌株中得以表达。细胞内荧光素酶的热稳定性因表达它的生物体而异，但与不同生物体的细胞耐热性无关。在加热培养基中添加木糖醇可防止细胞活力丧失和细胞内荧光素酶失活。甘油也可防止细胞活力丧失，但在防止荧光素酶热变性方面效果较差。
