Young J M, Triggs C M
Manaaki Whenua-Landcare Research, Auckland, New Zealand.
J Appl Bacteriol. 1994 Aug;77(2):195-207. doi: 10.1111/j.1365-2672.1994.tb03064.x.
The utility of 36 presumptive determinative tests for 32 pathovars of Pseudomonas syringae was investigated. A total of 395 strains was examined. Most strains of 12 of these pathovars (Ps. syringae pv. cannabina, Ps. syr. delphinii, Ps. syr. glycinea, Ps. syr. helianthi, Ps. syr. lachrymans, Ps. syr. mori, Ps. syr. morsprunorum, Ps. syr. phaseolicola, Ps. syr. 'porri', Ps. syr. papulans, Ps. syr. savastanoi and Ps. syr. tabaci) formed clusters when test data were compared by centroid analysis. Pseudomonas syr. syringae, Ps. syr. aptata, Ps. syr. atrofaciens, Ps. syr. dysoxyli and Ps. syr. japonica formed a single cluster, indicating their possible synonymy. Strains of Ps. syr. antirrhini and Ps. syr. tomato were indistinguishable, as were those of Ps. syr. garcae and Ps. syr. oryzae. Strains of Ps. syr. berberidis, Ps. syr. coronafaciens, Ps. syr. eriobotryae, Ps. syr. maculicola, Ps. syr. passiflorae, Ps. syr. pisi and Ps. syr. striafaciens and Ps. syr. tagetis did not form distinguishable clusters. The tests which reliably differentiated pathovars are recorded in a determinative scheme.
对丁香假单胞菌32个致病变种的36种推测性鉴定试验的效用进行了研究。共检测了395株菌株。通过质心分析比较测试数据时，其中12个致病变种（丁香假单胞菌大麻致病变种、丁香假单胞菌德尔菲尼致病变种、丁香假单胞菌大豆致病变种、丁香假单胞菌向日葵致病变种、丁香假单胞菌黄瓜角斑致病变种、丁香假单胞菌桑致病变种、丁香假单胞菌李痘致病变种、丁香假单胞菌菜豆致病变种、丁香假单胞菌葱致病变种、丁香假单胞菌丘疹致病变种、丁香假单胞菌萨瓦斯坦诺致病变种和丁香假单胞菌烟草致病变种）的大多数菌株形成了聚类。丁香假单胞菌丁香致病变种、丁香假单胞菌甜菜致病变种、丁香假单胞菌变黑致病变种、丁香假单胞菌染木树致病变种和丁香假单胞菌日本致病变种形成了一个单一聚类，表明它们可能是同物异名。丁香假单胞菌金鱼草致病变种和丁香假单胞菌番茄致病变种的菌株无法区分，丁香假单胞菌加西亚致病变种和丁香假单胞菌稻致病变种的菌株也是如此。丁香假单胞菌小檗致病变种、丁香假单胞菌燕麦晕斑致病变种、丁香假单胞菌枇杷致病变种、丁香假单胞菌斑点致病变种、丁香假单胞菌西番莲致病变种、丁香假单胞菌豌豆致病变种、丁香假单胞菌条纹致病变种和丁香假单胞菌万寿菊致病变种的菌株没有形成可区分的聚类。可靠区分致病变种的试验记录在一个鉴定方案中。
