Uner A H, Hutchison R E, Davey F R
Department of Pathology, State University of New York Health Science Center, Syracuse, New York.
Hematol Oncol Clin North Am. 1994 Aug;8(4):771-84.
In situ hybridization is a molecular technique that can be useful in the study of hematologic malignancies. Thus far, the most extensive uses have been in the detection of viral sequences and in the detection of chromosomal alterations. Other applications such as the identification of various cellular gene products are more limited, but with improvements in the sensitivity and reliability of methods for ISH, their use will become more widespread. Information provided by ISH contributes to our understanding of the pathophysiology and etiology of hematologic malignancies. In the future, ISH may be used routinely in the evaluation of leukemias and lymphomas.
原位杂交是一种分子技术，在血液系统恶性肿瘤的研究中可能有用。迄今为止，其最广泛的用途是检测病毒序列和染色体改变。其他应用，如鉴定各种细胞基因产物，则较为有限，但随着原位杂交方法的灵敏度和可靠性的提高，其应用将变得更加广泛。原位杂交提供的信息有助于我们理解血液系统恶性肿瘤的病理生理学和病因学。未来，原位杂交可能会常规用于白血病和淋巴瘤的评估。
