Johnson R E, Prakash S, Prakash L
Department of Biophysics, University of Rochester, New York.
J Biol Chem. 1994 Nov 11;269(45):28259-62.
The RAD5 gene of Saccharomyces cerevisiae functions in postreplication repair of ultraviolet damaged DNA, and interestingly, it also has a role in increasing the instability of simple repetitive sequences in the genome. In contrast to DNA mismatch repair genes which function in maintaining constant length of repeat sequences, RAD5 promotes alterations in the length of repeat sequences. In this work, we purify the RAD5 protein to near homogeneity from yeast cells and show that it is a single-stranded DNA-dependent ATPase. The possible roles of RAD5 ATPase in postreplication gap filling and in increasing the incidence of length alterations of repeat sequences are discussed.
酿酒酵母的RAD5基因在紫外线损伤DNA的复制后修复中发挥作用，有趣的是，它在增加基因组中简单重复序列的不稳定性方面也有作用。与在维持重复序列恒定长度中起作用的DNA错配修复基因不同，RAD5促进重复序列长度的改变。在这项工作中，我们从酵母细胞中纯化RAD5蛋白至近乎同质，并表明它是一种单链DNA依赖性ATP酶。讨论了RAD5 ATP酶在复制后缺口填补以及增加重复序列长度改变发生率方面的可能作用。
