艾滋病患者精子中的HIV颗粒及其向卵母细胞的转移。
HIV-particles in spermatozoa of patients with AIDS and their transfer into the oocyte.
作者信息
Baccetti B, Benedetto A, Burrini A G, Collodel G, Ceccarini E C, Crisà N, Di Caro A, Estenoz M, Garbuglia A R, Massacesi A
机构信息
Institute of General Biology, Consiglio Nazionale delle Ricerche, Siena, Italy.
出版信息
J Cell Biol. 1994 Nov;127(4):903-14. doi: 10.1083/jcb.127.4.903.
Abstract
By immunocytochemistry and in situ hybridization at the electron microscopy level, and by the PCR technique, we have shown that HIV-1 binds and enters normal sperm; that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer HIV-1 like particles to normal human oocytes. We also present evidence that a galactosylceramide-like compound is present on the sperm membrane and could function as an alternative receptor for HIV.
摘要
通过免疫细胞化学、电子显微镜水平的原位杂交以及聚合酶链反应技术，我们已经证明，HIV-1能够结合并进入正常精子；HIV-1感染男性的精子中存在病毒颗粒、其抗原和核酸；并且这种精子能够将HIV-1样颗粒转移至正常人类卵母细胞。我们还提供了证据表明，精子膜上存在一种半乳糖神经酰胺样化合物，它可能作为HIV的替代受体发挥作用。
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