与Vero细胞单层共培养可维持弱精子症精液样本的活力。

Co-culture with Vero cell monolayer maintains the motility of asthenozoospermic semen samples.

作者信息

Chen H F, Ho H N, Chen S U, Lien Y R, Chao K H, Lin H R, Huang S C, Lee T Y, Yang Y S

机构信息

Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan.

出版信息

Hum Reprod. 1994 Jul;9(7):1276-80. doi: 10.1093/oxfordjournals.humrep.a138694.

DOI:10.1093/oxfordjournals.humrep.a138694

PMID:7962433

Abstract

The clinical effectiveness of co-culture with Vero (Green monkey kidney) cell monolayer in maintaining the motility and viability of fresh asthenozoospermic semen (18 samples) and frozen-thawed semen with poor motility (motility fraction < 50%) (15 samples) in a 24-h period was evaluated. Co-culture with Vero cell monolayer in human tubal fluid (HTF) medium for 24 h resulted in a statistically better maintenance of motility percentage (P < 0.005), mean amplitude of lateral head displacement (ALH) (P < 0.005), and mean track speed (VCL) (P < 0.05) than culture in HTF medium alone. However, these motility parameters (motility percentage, ALH, VCL) declined soon after removal of spermatozoa from the monolayer. Co-culture with Vero cell monolayer also maintained the viability percentage of these sperm samples (52% of the original value) after the 24-h period compared with culture in HTF medium alone (22% of the original) (52% versus 22%, P < 0.05). It is concluded that Vero cell monolayer is effective in the maintenance of motility and viability of asthenozoospermic semen or frozen-thawed semen with poor motility. This co-culture system may be beneficial in enhancing the in-vitro performance of asthenozoospermic semen samples in the practice of assisted reproductive technology. However, its safety needs further evaluation.

摘要

评估了与Vero（绿猴肾）细胞单层共培养在24小时内维持新鲜弱精子症精液（18份样本）以及活力差的冻融精液（活力部分<50%）（15份样本）的活力和生存能力的临床效果。在人输卵管液（HTF）培养基中与Vero细胞单层共培养24小时，与仅在HTF培养基中培养相比，在活力百分比（P<0.005）、平均头部侧摆幅度（ALH）（P<0.005）和平均轨迹速度（VCL）（P<0.05）的维持方面具有统计学上更好的效果。然而，从单层中取出精子后，这些活力参数（活力百分比、ALH、VCL）很快下降。与仅在HTF培养基中培养相比（原始值的22%），与Vero细胞单层共培养在24小时后也维持了这些精子样本的生存能力百分比（原始值的52%）（52%对22%，P<0.05）。结论是，Vero细胞单层在维持弱精子症精液或活力差的冻融精液的活力和生存能力方面是有效的。这种共培养系统可能有助于在辅助生殖技术实践中提高弱精子症精液样本的体外性能。然而，其安全性需要进一步评估。