Tatke M, Doyle D
Department of Neuropathology, Southern General Hospital, Glasgow.
J Clin Pathol. 1994 Aug;47(8):774-5. doi: 10.1136/jcp.47.8.774.
Teased fibre preparations are important in the investigation of peripheral nerve disorders, but can be a rather tedious procedure. A technique is described in which pretreatment of the nerve sample with collagenase before osmication substantially simplifies the actual teasing procedure. The technique also does not alter the morphology or interfere with any further investigations required on teased fibres--for example, fluorescent antibody studies.
teased纤维制备在周围神经疾病的研究中很重要，但可能是一个相当繁琐的过程。本文描述了一种技术，即在锇酸固定之前用胶原酶对神经样本进行预处理，这大大简化了实际的 teased过程。该技术也不会改变形态，也不会干扰对 teased纤维所需的任何进一步研究，例如荧光抗体研究。
