产生IgG2b的小鼠骨髓瘤细胞系中产生IgG2a的变体。
IgG2a-producing variants of an IgG2b-producing mouse myeloma cell line.
作者信息
Francus T, Dharmgrongartama B, Campbell R, Scharff M D, Birshtein B K
出版信息
J Exp Med. 1978 Jun 1;147(6):1535-50. doi: 10.1084/jem.147.6.1535.
Abstract
12 variant cell lines producing an IgG2a (kappa) immunoglobulin derived via different routes from the IgG2b (kappa) synthesizing MPC 11 were studied. These variants all have the parental MPC 11 idiotype as shown by a radioimmunoassay. A comparison of the variants by charge, peptide maps, and assembly patterns has shown that most of them differ from one another, and some can be grouped. One group consists of three primary variants generated with two mutagenic agents: these three have almost indistinguishable peptide maps. Two other primary variants which arose in a similar fashion differ markedly from each other and from this group. A second group is comprised of the four secondary variants which arose from two short heavy chain producing primary variants. Other secondary variants and the one spontaneously arising variant cannot be grouped. Possible genetic mechanisms such as translocation, expression of previously silent genes and recombination are discussed.
摘要
研究了12种变异细胞系，它们通过不同途径从合成IgG2b（κ）的MPC 11产生IgG2a（κ）免疫球蛋白。如放射免疫测定所示，这些变异体均具有亲本MPC 11独特型。通过电荷、肽图和组装模式对这些变异体进行比较表明，它们中的大多数彼此不同，有些可以分组。一组由用两种诱变剂产生的三个主要变异体组成：这三个变异体的肽图几乎无法区分。以类似方式产生的另外两个主要变异体彼此之间以及与该组明显不同。第二组由从两个产生短重链的主要变异体产生的四个次要变异体组成。其他次要变异体和一个自发产生的变异体无法分组。讨论了可能的遗传机制，如易位、先前沉默基因的表达和重组。
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