Pazzaglia G, Winoto I, Jennings G
U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia.
J Diarrhoeal Dis Res. 1994 Jun;12(2):108-12.
The lack of an animal model for Aeromonas-associated diarrhoea has hindered progress toward understanding the pathogenesis of this potentially important enteric infection. Protein-malnourished mice were challenged orally with Aeromonas strains to determine if diminished levels of resistance would allow the induction of a diarrhoeal response. The 15 Aeromonas spp. faecal isolates used for challenge included 7 A. caviae, 4 A. hydrophila, 1 A. sobria bv. sobria, and 1 A. sobria bv. veronii from patients with diarrhoea, and 2 A. caviae from healthy volunteers. All had at least 1 known virulence marker, with the exception of 1 strain. Mice on the protein deficient diet had lost an average of 23% of their initial body weight at the time of challenge. Although mice consumed 10(8) cfu per day for a minimum of 4 days, none became ill due to Aeromonas spp. ingestion. Aeromonas spp. were isolated from 75% of faecal cultures obtained 7 days after initial challenge, indicating bowel colonization had occurred.
缺乏气单胞菌相关腹泻的动物模型阻碍了我们对这种潜在重要肠道感染发病机制的理解。对蛋白质营养不良的小鼠进行气单胞菌菌株口服攻击，以确定抵抗力降低是否会引发腹泻反应。用于攻击的15株气单胞菌粪便分离株包括7株豚鼠气单胞菌、4株嗜水气单胞菌、1株温和气单胞菌温和生物变种、1株温和气单胞菌维罗尼生物变种（来自腹泻患者）以及2株来自健康志愿者的豚鼠气单胞菌。除1株外，所有菌株均至少有1种已知毒力标记。在攻击时，蛋白质缺乏饮食的小鼠平均体重减轻了初始体重的23%。尽管小鼠每天摄入至少4天的10(8) cfu，但没有一只因摄入气单胞菌而生病。在初次攻击7天后获得的粪便培养物中，75%分离出气单胞菌，表明已发生肠道定植。
