Lapiere J C, Chen J D, Iwasaki T, Hu L, Uitto J, Woodley D T
Department of Dermatology, Northwestern University School of Medicine, Chicago, Illinois 60611.
J Invest Dermatol. 1994 Nov;103(5):637-41. doi: 10.1111/1523-1747.ep12398270.
Type VII collagen is the major component of anchoring fibrils, structures within basement membranes beneath stratified squamous epithelium thought to mediate the adherence of the epidermis to the dermis of human skin. Type VII collagen has affinity for fibronectin. The interaction between type VII collagen and fibronectin is mediated through the collagen-binding domain on the amino terminus of fibronectin. Heretofore, the domain on the type VII collagen molecule that binds to fibronectin was not known. In this study, we mapped the binding site of fibronectin to a specific subdomain of the triple helical collagenous region of type VII collagen, immediately adjacent to the small carboxyl terminal non-collagenous domain. This fibronectin-binding site within the type VII collagen molecule lies between nucleotide residues 615 and 1161.
VII型胶原蛋白是锚定原纤维的主要成分，锚定原纤维是复层鳞状上皮下方基底膜内的结构，被认为介导人类皮肤表皮与真皮的黏附。VII型胶原蛋白对纤连蛋白具有亲和力。VII型胶原蛋白与纤连蛋白之间的相互作用是通过纤连蛋白氨基末端的胶原结合域介导的。迄今为止，VII型胶原蛋白分子上与纤连蛋白结合的结构域尚不清楚。在本研究中，我们将纤连蛋白的结合位点定位到VII型胶原蛋白三螺旋胶原区域的一个特定亚结构域，该亚结构域紧邻小的羧基末端非胶原结构域。VII型胶原蛋白分子内的这个纤连蛋白结合位点位于核苷酸残基615和1161之间。
