Gallay P, Barras C, Tobias P S, Calandra T, Glauser M P, Heumann D
Department of Immunology, Scripps Clinic, La Jolla, California.
J Infect Dis. 1994 Nov;170(5):1319-22. doi: 10.1093/infdis/170.5.1319.
Lipopolysaccharide (LPS)-binding protein (LBP) and CD14 represent key elements in monocyte activation by LPS. The mean concentration of LBP was 18.1 microgram/mL in normal serum and 40-60 micrograms/mL in serum of patients with septic shock, independent of the fact that patients had gram-negative or other infections. Ten percent normal serum presented large concentrations of LPS (in the microgram range) to monocytes. Only when diluted 1:100 was LBP in plasma a limiting factor for monocyte activation, as measured by tumor necrosis factor (TNF) release. When LBP was depleted from serum with anti-LBP antibodies, the resulting serum did not support TNF release of monocytes upon LPS challenge. In conclusion, monocyte activation resulting in TNF secretion was related to LBP, which is abundantly present in normal serum, and elevated two to three times in patients with septic shock.
脂多糖（LPS）结合蛋白（LBP）和CD14是LPS激活单核细胞的关键因素。正常血清中LBP的平均浓度为18.1微克/毫升，脓毒性休克患者血清中为40 - 60微克/毫升，与患者是革兰氏阴性菌感染还是其他感染无关。10%的正常血清会向单核细胞呈现高浓度的LPS（微克级别）。只有当血浆中的LBP稀释1:100时，才会成为单核细胞激活的限制因素，这是通过肿瘤坏死因子（TNF）释放来衡量的。当用抗LBP抗体从血清中去除LBP后，所得血清在LPS刺激下不能支持单核细胞释放TNF。总之，导致TNF分泌的单核细胞激活与LBP有关，LBP在正常血清中大量存在，在脓毒性休克患者中升高两到三倍。
