Turek J J, Schoenlein I A, Clark L K, Van Alstine W G
Department of Veterinary Anatomy, Purdue University School of Veterinary Medicine, West Lafayette, Indiana 47907-1242.
J Leukoc Biol. 1994 Nov;56(5):599-604.
The effects of various dietary polyunsaturated fatty acids (PUFAs) on the function of immune cells of the porcine lung was studied. Groups of six pigs were fed diets containing 10.5% corn oil [CO; enriched in linoleic acid (18:2, n-6)], linseed oil (LO; enriched in alpha-linolenic acid (18:3, n-3)], menhaden oil (MO; enriched in eicosapentaenoic (20:5; n-3) and docosahexaenoic (22:6; n-3) acids], linseed + corn oil (1:1; LC), and menhaden + corn oil (1:1; MC) for 28-30 days. Basal levels of alveolar macrophage (m phi) tumor necrosis factor-alpha (TNF-alpha) production were higher (P < .05) for LC- and MC-fed pigs than for CO- and LO-fed pigs. Lipopolysaccharide (LPS)-stimulated LC and MC m phi s produced more TNF than m phi s from pigs fed CO, LO, and MO diets. Macrophages from pigs receiving the CO and LC diets had higher (P < .05) levels of leucine aminopeptidase than m phi s from the other dietary groups. Lipopolysaccharide did not increase m phi nitrite production over basal levels except in the MO diet group. However, LPS-stimulated m phi s from the CO, MO, and LC dietary groups produced more nitrite than m phi s from MC-fed pigs. Alveolar lymphocytes from pigs receiving the MC diet produced more T cell growth factors than LO and MO m phi s. Alveolar m phi s from the different dietary groups did not differ in their capacity for non-immune-mediated phagocytosis of fluorescent latex beads. These results indicate that dietary PUFAs can modulate some functions of porcine alveolar immune cells and that this may prove significant for host response to respiratory disease agents.
研究了各种膳食多不饱和脂肪酸（PUFA）对猪肺免疫细胞功能的影响。将六头猪分为几组，分别喂食含10.5%玉米油[CO；富含亚油酸（18:2，n-6）]、亚麻籽油（LO；富含α-亚麻酸（18:3，n-3）]、鲱鱼油（MO；富含二十碳五烯酸（20:5；n-3）和二十二碳六烯酸（22:6；n-3）]、亚麻籽+玉米油（1:1；LC）和鲱鱼+玉米油（1:1；MC）的日粮28 - 30天。与喂食CO和LO日粮的猪相比，喂食LC和MC日粮的猪肺泡巨噬细胞（m phi）肿瘤坏死因子-α（TNF-α）的基础产生水平更高（P < 0.05）。脂多糖（LPS）刺激的LC和MC m phi产生的TNF比喂食CO、LO和MO日粮的猪的m phi更多。接受CO和LC日粮的猪的巨噬细胞中亮氨酸氨肽酶水平比其他日粮组的m phi更高（P < 0.05）。除MO日粮组外，脂多糖不会使m phi亚硝酸盐产生量超过基础水平。然而，LPS刺激的来自CO、MO和LC日粮组的m phi产生的亚硝酸盐比喂食MC日粮的猪的m phi更多。接受MC日粮的猪的肺泡淋巴细胞产生的T细胞生长因子比LO和MO m phi更多。不同日粮组的肺泡m phi对荧光乳胶珠的非免疫介导吞噬能力没有差异。这些结果表明，膳食PUFA可以调节猪肺泡免疫细胞的某些功能，这可能对宿主对呼吸道疾病病原体的反应具有重要意义。
