Reinisch N, Sitte B A, Kähler C M, Wiedermann C J
Department of Internal Medicine, University of Innsbruck, Austria.
J Endocrinol. 1994 Jul;142(1):167-70. doi: 10.1677/joe.0.1420167.
Treatment of rats with human chorionic gonadotrophin (hCG) induced in the testes an inflammation-like reaction characterized by migration of leukocytes into the interstitial space. In order to find out whether hCG acts in a direct manner in this process, we tested peripheral human blood leukocyte attraction by hCG in vitro. Chemotaxis through cellulose nitrate to gradients of test substances was measured using a 48-well microchemotaxis chamber. Human CG was found to be a potent attractor of neutrophils, monocytes and lymphocytes in vitro in the picomolar concentration range. Checkerboard analyses revealed that the type of migration depends on positive concentration gradients of hCG. The chemoattractant nature of hCG is consistent with its having a role to play in regulation of tissue accumulation of these cells within the reproductive tract.
用人绒毛膜促性腺激素（hCG）处理大鼠可在睾丸中诱导出类似炎症的反应，其特征为白细胞迁移至间质间隙。为了弄清楚hCG在此过程中是否直接发挥作用，我们在体外测试了hCG对人外周血白细胞的吸引作用。使用48孔微量趋化性小室测量通过硝酸纤维素对测试物质梯度的趋化性。发现人绒毛膜促性腺激素（hCG）在皮摩尔浓度范围内是体外中性粒细胞、单核细胞和淋巴细胞的有效吸引剂。棋盘分析表明，迁移类型取决于hCG的正浓度梯度。hCG的化学吸引剂性质与其在生殖道内这些细胞的组织积聚调节中发挥作用是一致的。
