Yamada N, Tanihara K, Mizokami M, Ohba K, Takada A, Tsutsumi M, Date T
Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan.
J Gen Virol. 1994 Nov;75 ( Pt 11):3279-84. doi: 10.1099/0022-1317-75-11-3279.
Hepatitis C virus (HCV) type K3a (type 3a), which represents a minor genotype in Europe, the U.S.A. and Asia, appears to be significantly distributed throughout Australia and Brazil. We amplified the HCV-K3a/650 genome by reverse transcription polymerase chain reaction in ten overlapping fragments and determined the nucleotide sequences. The total sequence was 9454 bases in length and contained an open reading frame of 3021 amino acids, which is 10 or 11 amino acids longer than in HCV type 1 and 12 amino acids shorter than the sequence of type 2. These differences were due to the different lengths of both the putative envelope protein E2 and the NS5A regions, whose nucleotide lengths differ between types 1 and 2 also. Phylogenetic analysis of the putative core region and a portion of NS5B encoding the Gly-Asp-Asp motif indicated that HCV-K3a closely matched the corresponding type 3a group. The deletion and addition of amino acids in both E2 and NS5A may be associated with their pathobiological features.
丙型肝炎病毒（HCV）K3a型（3a型）在欧洲、美国和亚洲属于次要基因型，但在澳大利亚和巴西似乎分布广泛。我们通过逆转录聚合酶链反应将HCV-K3a/650基因组扩增为10个重叠片段，并测定了核苷酸序列。总序列长度为9454个碱基，包含一个3021个氨基酸的开放阅读框，比1型HCV长10或11个氨基酸，比2型序列短12个氨基酸。这些差异是由于假定的包膜蛋白E2和NS5A区域的长度不同，其核苷酸长度在1型和2型之间也有所不同。对假定的核心区域和编码甘氨酸-天冬氨酸-天冬氨酸基序的NS5B的一部分进行系统发育分析表明，HCV-K3a与相应的3a型组密切匹配。E2和NS5A中氨基酸的缺失和添加可能与其病理生物学特征有关。
