Redlich M, Peleg I, Cooperman H, Shoshan S
Connective Tissue Research Laboratory, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
J Periodontol. 1994 Aug;65(8):776-80. doi: 10.1902/jop.1994.65.8.776.
Collagen mRNA levels in the gingival cells of molars and incisors in rats were measured and correlated with the ratio of interstitial collagen to DNA in these regions. Hybridization of 32P-labeled specific cDNA probes for collagen types I and III with total RNA isolated from gingival tissue of rat molars and incisors showed that the steady-state levels of mRNAs of type I was significantly higher in the molars than in the incisors (molars/incisors = 2.12 +/- 0.12, P < 0.004). However, the ratio of interstitial collagen to DNA in the gingiva of the molars was significantly lower than that found in the incisors (collagen/DNA = 4.13 +/- 0.90 and 12.89 +/- 1.24 respectively, P < 0.001). It is suggested that the difference between the mRNA levels and those of interstitial collagen may reflect an intrinsic characteristic presumably associated with the different modes of mastication between molars and incisors of the rat.
测量了大鼠磨牙和切牙牙龈细胞中胶原蛋白mRNA水平，并将其与这些区域间质胶原蛋白与DNA的比例相关联。用32P标记的I型和III型胶原蛋白特异性cDNA探针与从大鼠磨牙和切牙牙龈组织中分离的总RNA进行杂交，结果显示I型mRNA的稳态水平在磨牙中显著高于切牙（磨牙/切牙 = 2.12 +/- 0.12，P < 0.004）。然而，磨牙牙龈中间质胶原蛋白与DNA的比例显著低于切牙（胶原蛋白/DNA分别为4.13 +/- 0.90和12.89 +/- 1.24，P < 0.001）。这表明mRNA水平与间质胶原蛋白水平之间的差异可能反映了一种内在特征，推测与大鼠磨牙和切牙不同的咀嚼方式有关。
