Ling L J, Lai Y H, Hwang H, Chen H
Division of Periodontics, Veterans General Hospital-Taipei, School of Dentistry, National Yang Ming Medical College, Taiwan, R.O.C.
J Periodontol. 1994 Aug;65(8):781-7. doi: 10.1902/jop.1994.65.8.781.
This study was designed to determine the histologic response of regenerated tissue to plaque, using an adult Taiwan monkey model with chronically-inflamed, surgically-created, periodontal defects. Standardized 2-walled periodontal defects were surgically created at the mesial of the mandibular lateral incisor on one side. Sutures, 3-0 braided silk, were placed for 8 weeks and tissues positioned so that denuded root surfaces were exposed to plaque, with the subsequent development of the chronically-inflamed periodontal tissues. At 8 weeks the defects were subjected to guided tissue regeneration procedures using expanded polytetrafluoroethylene membrane and allowed to heal for 6 months. Contralateral clinically healthy sites, without surgical procedures, were used as controls. In both experimental (membrane) and control sites, silk ligatures were placed to allow plaque accumulation for 2 or 10 weeks. Four monkeys were sacrificed at each time point and specimens processed for histologic and histometric study. The results indicated that plaque-induced inflammation was less at sites treated by guided tissue regenerative procedures when compared to originally clinically-healthy sites exposed to plaque for the same duration. However, histologically the newly formed osseous tissue was compromised under these conditions.
本研究旨在利用成年台湾猴模型，通过手术制造慢性炎症性牙周缺损，来确定再生组织对菌斑的组织学反应。在一侧下颌侧切牙近中处手术制造标准化的两壁牙周缺损。用3-0编织丝线缝合8周，并对组织进行定位，使裸露的根面暴露于菌斑，随后形成慢性炎症性牙周组织。8周时，使用膨体聚四氟乙烯膜对缺损部位进行引导组织再生手术，并使其愈合6个月。对侧未经手术的临床健康部位用作对照。在实验（使用膜）部位和对照部位，均放置丝线结扎以允许菌斑积聚2周或10周。在每个时间点处死4只猴子，并对标本进行组织学和组织计量学研究。结果表明，与相同时间暴露于菌斑的原本临床健康部位相比，引导组织再生手术治疗的部位菌斑诱导的炎症较轻。然而，在这些条件下，组织学上新形成的骨组织受到了损害。
