Morehead M C, Franklin W, Fu P P, Evans F E, Heinze T M, Cerniglia C E
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
J Toxicol Environ Health. 1994 Nov;43(3):369-80. doi: 10.1080/15287399409531928.
Pure cultures of anaerobic intestinal bacteria and mixed fecal microflora from human, rat, mouse, and pig were screened for the ability to metabolize 7-nitrobenz[a]anthracene (7-NO2BA). Based on analysis by high-performance liquid chromatography (HPLC) and by ultraviolet (UV), mass, and nuclear magnetic resonance (NMR) spectral techniques, the compounds were identified as 7-aminobenz[a]anthracene (7-NH2BA) and benz[a]anthracene 7,12-dione (dione). Identification of 7-NH2BA as a metabolite of 7-NO2BA indicates that the anaerobic intestinal bacteria are capable of reducing 7-NO2BA to potentially bioactive intermediates. The reductive capacities of the mixed intestinal microflora were generally greater than those of pure cultures. Thus, metabolism of 7-NO2BA in the intestinal tract may be underestimated if pure cultures are used as the sole method for evaluating the potential hazard.
对来自人类、大鼠、小鼠和猪的厌氧肠道细菌纯培养物以及混合粪便微生物群进行筛选，以检测它们代谢7-硝基苯并[a]蒽（7-NO2BA）的能力。基于高效液相色谱（HPLC）以及紫外（UV）、质谱和核磁共振（NMR）光谱技术的分析，这些化合物被鉴定为7-氨基苯并[a]蒽（7-NH2BA）和苯并[a]蒽7,12-二酮（二酮）。将7-NH2BA鉴定为7-NO2BA的代谢产物表明，厌氧肠道细菌能够将7-NO2BA还原为潜在的生物活性中间体。混合肠道微生物群的还原能力通常大于纯培养物。因此，如果仅使用纯培养物作为评估潜在危害的唯一方法，肠道中�-NO2BA的代谢情况可能会被低估。
